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STABILIZATION OF NADPH OR NADH IN AMMONIA DETECTION ASSAYS
STABILIZATION OF NADPH OR NADH IN AMMONIA DETECTION ASSAYS
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机译:氨检测测定中NADPH或NADH的稳定化
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摘要
The present disclosure deals with the biochemistry of reagents useful in the detection of ammonia in liquid samples. Specifically, the present disclosure is directed to a technical improvement of an enzyme-based test for ammonia that can be used for analysis of plasma samples taken from patients in clinical settings, among other uses. In this regard, stability of a reagent containing NAD(P)H is improved, enhancing shelf life and results in the detection of ammonia. In an exemplary reagent ammonia released as a result of NAD(P)H decay is scavenged using an enzymatic reaction to convert the ammonia using GLDH, NAD(P)H and 2-oxoglutarate, thereby forming L-glutamate, NAD(P)+ and Η2O in the NAD(P)H containing reagent.
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