Extraction and Purification of DNA polymerase from Geobacillus lituanicus with properties similar to Bst DNA polymerase

摘要

The present invention compares and infers the protein sequence of DNA polymerase I extracted from Geobacillus lituanicus with Bst DNA polymerase extracted from Bacillus stearothermophilus, and the Geobacillus lituanicus (Geobacillus lituanicus), biosynthesis of 1,749 bases corresponding to 583 amino acids of C-terminus related to the Bst DNA polymerase in DNA polymerase I, and cloning the biosynthesis result into a bacterial expression vector It relates to a DNA polymerase extracted and purified from Geobacillus lithuanicus, characterized in that it has properties similar to that of Bst DNA polymerase. According to the present invention, through the securing of a new resource by extraction of DNA polymerase from a new Bacillus species to have similar efficacy to Bst DNA polymerase extracted from Bacillus stearomophilus, instead of Bst DNA polymerase It has the effect of providing a DNA polymerase that can be usefully used when performing isothermal PCR.