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GENE ENCODING A THERMOSTABLE DNA POLYMERASE FROM THERMUS AQUATICUS,SAID DNA POLYMERASE AND ITS PURIFICATION
GENE ENCODING A THERMOSTABLE DNA POLYMERASE FROM THERMUS AQUATICUS,SAID DNA POLYMERASE AND ITS PURIFICATION
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机译:编码来自水生栖热菌的可热DNA聚合酶的基因,有用的DNA聚合酶及其纯化
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摘要
Recombinant DNA sequences encoding a thermostable DNA polymerase from Thermus aquaticus can be used to produce a recombinant protein with a molecular weight of about 86,000-95,000 daltons. The thermostable recombinant enzyme can be used in a temperature-cycling chain reaction wherein at least one nucleic acid sequence is amplified in quantity from an existing sequence with the aid of selected primers and nucleotide triphosphates. The enzyme is preferably stored in a buffer containing non-ionic detergents that lends stability to the enzyme.
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