A high-throughput screening method for a non-target biomarker employing pollutant metabolic turbulence, pertaining to the field of environmental exposure and health. The method comprises the steps of: (1) performing extraction to obtain an extract to be tested; (2) performing chromatographic analysis to obtain a spectrogram containing a chromatographic peak; (3) identifying and marking a pollutant characteristic peak, and using a chromatographic peak other than the pollutant characteristic peak as a potential metabolite characteristic peak, and performing non-target marking on the potential metabolite characteristic peak; (4) using the peak area of the potential metabolite characteristic peak as a dependent variable, and the peak area of the pollutant characteristic peak as an independent variable, so as to establish a linear regression model; (5) running the model to perform non-target screening of a biomarker to initially obtain a relevant biomarker; and (6) analyzing primary and secondary spectrograms of the biomarker initially obtained, so as to identify a biomarker related to pollutant exposure. The present invention significantly improves the accuracy of biomarker screening, and at the same time increases the throughput of biomarker screening.
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