Signal to noise increase in nucleic acid sequencing

摘要

(a) providing a plurality of primers - template nucleic acid hybridsThe primer has a stretchable 3 'endProviding(b)(I) primers containing nucleotides blocked at 3 'ends - blocked nucleotides for generating a first subset of template nucleic acid hybridsAnd (II) primers containing ternary complex inhibitors - contacting a ternary complex inhibitor for generating a second subset of template nucleic acid hybrids(c) each is polymeraseFirst subset primer - template nucleic acid hybridsForming a ternary complex containing homologous nucleotides andAnd (d) the ternary complex is detected.Thereby, by identifying nucleotides in the template nucleic acidA method for identifying nucleotides in a template nucleic acid.No selection