METHOD FOR CREATING A GENOMIC LIBRARY ENRICHED FOR BACILLUS AND IDENTIFICATION OF NOVEL CRY TOXINS

摘要

The disclosure is directed to an approach for discovering novel insecticidal proteins from highly heterogeneous environmental sources. The methodology utilizes metagenomic enrichment procedures that increase the representation of specific genera of bacteria, prior to deep sequencing, in order to access novel variants of insecticidal proteins. In aspects, the taught methods are useful for creating large genomic libraries enriched for Bacillus sp. The enriched genomic libraries are useful for the discovery of novel cry toxin genes, which enable the identification of novel Cry proteins.