Problem to be solved: to provide a method for evaluating the microbial contamination of the organism which can detect the contamination of the microbial contamination, especially the contamination by the microorganism holding the metabolic activity, especially the presence of the biofilm contamination.CAndContacting the test liquid containing at least one stable isotope selected from the group consisting of N andThe ratio of the stable isotopes at the surface of the object after the contact is obtained in two dimensions using the secondary ion mass spectrometryIn the measurement rangeComparing the existence ratio with the natural abundance ratio of the stable isotopeThe concentration of the stable isotopes is calculated in S602 andIf the enrichment state exceeds a predetermined criterionIt is to be noted that s604 provides information for determining that microbial contamination is occurring in the measurement rangeEvaluation method of microbial contamination of objects including.Diagram
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