METHOD FOR DETECTING TARGET NUCLEIC ACID, METHOD FOR DETECTING NUCLEIC ACID-BINDING MOLECULE, AND METHOD FOR EVALUATING NUCLEIC ACID-BINDING ABILITY

摘要

The present invention is a method for detecting a target nucleic acid, in which the target nucleic acid can be detected distinctively from a non-target nucleic acid for which the nucleotide sequence or modification state is partially different from that for the target nucleic acid, the method comprising: performing a nucleic acid amplification reaction using a region in the non-target nucleic acid which is different from a corresponding region in the target nucleic acid as a corresponding target region and a region in the target nucleic acid which is different from a corresponding region in the non-target nucleic acid as a target region, also using a nucleic acid sample of interest as a template, and also using a primer capable of hybridizing with both of the target nucleic acid and the non-target nucleic acid in the presence of a molecule capable of binding specifically to the target region in the non-target nucleic acid under temperature conditions under which the molecule can bind to the non-target nucleic acid; and detecting the target nucleic acid on the basis of the presence or absence of an amplification product.