Primer sets for diagnosing of new and variant sweet potato viruses and diagnostic methods using thereof

摘要

The present invention is one or more primers selected from the group consisting of a primer pair represented by SEQ ID NO: 1 and SEQ ID NO: 2, a primer pair represented by SEQ ID NO: 3 and SEQ ID NO: 4, and a primer pair represented by SEQ ID NO: 5 and SEQ ID NO: 6 A set of primers for diagnosing a sweet potato virus selected from the group consisting of a novel sweet potato virus SPVF, SPVE, and a variant sweet potato virus SPVG, including a pair, a composition for diagnosing a sweet potato virus comprising the primer pair, a kit for diagnosing a sweet potato virus comprising the composition , And to a method for diagnosing sweet potato virus. In the present invention, two novel sweet potato viruses SPVF, SPVE and one strain of sweet potato virus SPVG were identified by creating a library from sweet potato leaves collected from 10 different regions and performing virome analysis. Alternatively, it is possible to improve the accuracy of diagnosis by designing a primer pair capable of selectively and specifically binding to each variant sweet potato virus, and thus it can be usefully used for monitoring sweet potato infection virus and development of virus free sweet potato.