Microscope objective with low magnification for epi-microscopes

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• 3. MICROSCOPE OBJECTIVE LENS FOR EXTREMELY LOW MAGNIFICATION AND MICROSCOPE [P] . 日本专利： JPH10161035A . 1998-06-19

  机译： 极低放大倍率和显微镜的显微镜物镜

• 4. PLANAHROMATICHESKY low magnification microscope objective [P] . 俄罗斯专利： RU66558U1 . 2007-09-10

  机译： PLANAHROMATICHESKY低倍显微镜物镜

• 5. Stereo optical microscope for intraocular surgery has main objective followed by magnification system with perpendicualr optical axis [P] . 德国专利： DE10332602B3 . 2005-01-05

  机译： 用于眼内手术的立体光学显微镜的主要目的是其后是具有垂直光轴的放大系统

• 1. Micro Flow-Through Thermocycler with Simple Meandering Channel with Symmetric Temperature Zones for Disposable PCR-Devices in Microscope Slide Format [J] . Anett Reichert, Jana Felbel, Mark Kielpinski, 仿生工程学报（英文版） . 2008,第004期
• 2. Image Similarity for Routine Nailfold Capillaroscopy by using Microscope with Low and High Magnification [C] . Hieyong Jeong, Yuko Ohno システム制御情報学会研究発表講演会 . 2019
• 3. Overview of the Microscope Objective [D] . Niu, Ruijuan. 2017
• 4. A noise reduction method for quantifying nanoparticle light scattering in low magnification dark-field microscope far-field images [O] . Dali Sun, Jia Fan, Chang Liu, -1
• 5. Loupe Magnification for Small Incision Cataract Surgery - an Alternative to Microscope Magnification? [O] . Sanjay Kumar Singh, I Winter, A Hennig 2008
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• 1. Observation of Cell-Size Variation under Environmental Stress by Fluorescence Correlation Spectroscopy without Objective Image Magnification [J] . Kitao Fujiwara ,Keishiro Horiuchi ,Sayuri Goryoda . 环境科学与工程：A . 2012,第003期
• 2. A Cosmic Microscope to Probe the Universe from Present to Cosmic Dawn:Dual-element Low-frequency Space VLBI Observatory [J] . AN Tao ,JAISWAL Sumit ,MOHAN Prashanth . 空间科学学报 . 2019,第002期
• 3. A Low Cost and Versatile STED Superresolution Fluorescent Microscope [J] . Daniel McBride ,Chin Su ,Jun Kameoka . 现代仪器（英文） . 2013,第3期
• 4. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 5. 连续变倍体视显微镜附加大物镜的设计 [C] . 张斌 . 2003年十一省（市）光学学术会议 . 2003
• 6. 高连续变倍比体视显微镜——变倍物镜的光学设计 [A] . 侯晓萍 . 2006