Orotidine production with pyrimidine requiring - strain of neurospora crassa

摘要

The mutation strain, pref. Neurospora crassa 36601, is cultured in an aqs. pyrimidine-containing medium, pref. at pH 3.5-5.5 (4-4.4), separated, and orotidine (N-riboside of 6-carboxyuracil), useful as intermediate in pyrimidine series synthesis reactions, is separated from the acid medium, pref. by adsorption on charcoal. Pref. culture medium contains over 0.01% weight/vol. Na ions and over 5 (30-50) mg/l. Zn ions.