METHOD FOR THE DETERMINATION OF FIBRIN SPLIT PRODUCTS AND FIBRINOGEN SPLIT PRODUCTS IN BLOOD SERUM

摘要

ABSTRACT OF THE DISCLOSUREA method for the determination of fibrin split products and fibri-nogen split products in blood serum which comprises: A. Adding a specifiedamount of an imidazole buffer to each well of a microtiter plate; B. Addingan amount equivalent to the amount of (A) of blood serum test sample to thefirst well of the microtiter plate of (A) and serially diluting, using amicrotiter diluter; C. Adding an amount equivalent to the amount of (A), ofa dyed Staphlococcus aureus cell suspension in the buffer of (A) to each wellof the microtiter plate of (B); and D. Observing the wells of (C) for visi-ble clumping of the Staphylococcus aureus cells as a positive indication ofthe presence of fibrin split products and fibrinogen split products in thetest blood serum. Coloration of the cells greatly improves visualization ofthe end point of the test. The concentration of fibrinogen split productsand/or fibrin split products in the blood serum test sample can be calculatedif a comparison test is run with a serum containing a known amount of fibrin-ogen split products and/or fibrin split products.