Establishment of the Determination Methods of Total Anthraquinone Content in Different Processed Products of Rhubarb

摘要

[Objectives]Ultraviolet spectrophotometry was established for the determination of total content of anthraquinone in different processed products of rhubarb.[Methods]Emodin was used as the control substance,and its color was developed by magnesium acetate methanol solution with the concentration of 1%,the absorbance was determined at the wavelength of 516 nm,the content of total anthraquinone in different processed products of rhubarb was determined to compare the content differences.[Results]The regression equation was y=0.0452 x+0.0015,R 2=0.9991.Emodin had a good linear range in the concentration of 0.98-23.52μg/mL,with high accuracy,good repeatability and stable detection results.This method revealed the content changes of total anthraquinone in rhubarb before and after processing.[Conclusions]This method could be used as the quality control method of different processed products of rhubarb,and provided reference for the comprehensive development and utilization of rhubarb resources in the future.