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Method of manufacture of the complex of microbiological nebramycin
Method of manufacture of the complex of microbiological nebramycin
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机译:微生物奈布霉素复合物的制造方法
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摘要
In a new process for the microbioglical prodn. of the nebramycin complex, (a) a strain of Streptomyces tenebrarius which biosynthesises the nebramycin complex is cultivated in a nutrient medium contg. nebramycin 2, nebramycin 5' and/or nebramycin 6 and the individuals forming growing aerial mycelium and spores in the presence of 10-30 mg ml of these antibiotics are isolated; (b) the resulting new resistant strains are cultivated in a submerged aerobic culture in a nutrient medium contg. N- and C- sources, inorganic salts and (opt.) vegetable and/or animal fats at 30-42 degrees C (pref. 35-38 degrees C); and the nebramycin complex or nebramycin 2, nebramycin 4 and nebramycin 5' accumulating in the medium is isolated. Nebramycin 2 (apramycin) is used in the treatment of diseases of animals and plants (cf. US 3691279, 3853709, 3876767). Nebramycin 6 (tobramycin), produced by hydrolysis of nebramycin 5' (6"-O-carbamoyl-tobramycin), is a broad-spectrum antibiotic used in human medicine, esp. against polyresistant Pseudomonas strains. Use of selected nebramycin-resistant Streptomyces tenebrarius strains allows the prodn. of cultures with high nebramycin concns. thus giving improved yields.
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