SUPPRESSIVE FACTOR OF ALLERGY

摘要

SUPPRESSIVE FACTOR OF ALLERGYAbstract of DisclosureA monoclonal hybridoma cell line was constructedfor the production of suppressive factor of allergy (SFA).mRNA isolated from the monoclonal hybridoma cell line wasidentified by its ability to direct SFA synthesis inXenopus laevis oocytes. The SFA produced by the monoclonalhybridoma cell line and the translational product of themRNA in the oocytes was found effective in inhibitingIgE- specific receptor expression in dilutions greaterthan 1:1,000 of the culture supernatant. A sensitivebioassay for SFA was developed using cultures ofthymocytes in which Lyt-2 cells were blocked withmonoclonal antibodies, and subsequent IgE-specificreceptor expression was determined in Lyt-1 cells.