ASSAY OF IGA ANTIBODY AGAINST INTESTINAL TRACT INFECTION DISEASE VIRUS IN WHEY AND SERUM OF MAMMAL

摘要

PURPOSE:To achieve a measurement handily, quickly and with a high sensitivity, by using virus refined from a supernatant in a culture of virus infected cell as immobilizing antigen while enzyme-labeled anti IgA antibody as labeled antibody. CONSTITUTION:Antigen is immobilized on a plate for ELISA by a carbonate buffer, left alone at one night and after washings five times by a washing liquid, a bovine serum alubmin PBS is added thereto to react. Furthermore, after washings five times by a washing liquid, a diluted sample is added to the mixture to react. After washings five times, a goat anti-pig alpha chain IgG diluted solution labeled by peroxide is added to the product to react. After washings five times by a washing liquid, citric acid/phosphoric acid buffer solution (containing H2O2) of o-phenylene diamine is added to the mixture to react. Then, a H2SO4 solution is added to the solution to stop the reaction and then, absorbance of 492nm is measured.