EVALUATION OF THE SENSITIVITY, SPECIFICITY AND TIME POST INFECTION FOR DETECTION OF TGE ANTIBODIES USING TWO COMMERCIAL BLOCKING ELISA ASSAYS COMPARED TO THE SERUM NEUTRALIZATION ASSAY

摘要

TGE infection is associated with diarrhea, vomiting and death of suckling pigs and also associated with mild diarrhea and vomiting of older pigs. TGE belongs to the coronaviridae family, which also includes PRCV. PRCV can cause respiratory clinical signs, but is mostly recognized for the cross protection and cross reaction on the SN test against TGE. The diagnosis of TGE can be done indirectly, with the detection of virus antibodies in serum using serum neutralization (SN) or blocking ELISA. For thisstudy the objectives were threefold. First to document the performances of two blocking ELISA assays relative to the SN test, secondly, to estimate the probability of a serum with high SN titer would test positive for TGE, and finally, to determine thenumber of days post inoculation required for each test to detect positive samples.