ALPHA-GALACTOSIDASE OF TARO,PREPARATION THEREOF AND TRANSFORMATION NECCESSARY FOR LIFE MAINTENANCE FROM BLOOD OF B TYPE TO BLOOD OF O TYPE USING IT

摘要

NEW MATERIAL: Alpha-galactosidase extracted and isolated from Colocasia esculenta. ;USE: Used as an enzyme for converting B-group red blood cells to 0group red blood cells. ;PREPARATION: Alpha-galactosidase of Colocasia esculenta is obtained for example, by crushing Colocasia esculenta in a weak acid buffer solution, filtering a suspension to a transparent solution, concentrating the solution by ultra filtration, collecting enzyme solution by column chromatography, concentrating the enzyme solution, collecting enzyme solution through cation exchange column chromatography, concentrating the solution and collecting enzyme solution through anion exchange column chromatography to purify the enzyme.;COPYRIGHT: (C)1990,JPO