Preparation of Fine Implantable Needle Type Biosensors for Blood Vessel Glucose Monitoring

摘要

Fine needle type glucose sensors with variety of outer membrane were prepared and their glucose sensor properties were evaluated by in vitro measurement in phosphate buffer solution and horse serum, and in vivo measurement using the veins of rats and rabbits. Extra-outer membrane materials of polyglutamic acid (PGA) and heparin, which provide hydrophilic surface were introduced in addition to polyurethane/polydimethylsiloxane composite outer membrane. Glucose sensors without extra-outer film and with PGA extra-outer film showed glucose response on the day of implantation, while clear response was not obtained on the second day of implantation due to the adsorption of thrombus on the sensor surface. On the other hand, the sensor with heparin outer membrane provided satisfactory glucose response also on the second day.