DNA EXHIBITING COMPLEMENTARITY TO GENE RNA OF HUMAN IMMUNODEFICIENCY VIRUS-2 TYPE

摘要

PURPOSE:To make possible to develop a vaccine and diagnostic, etc., of a DNA exhibiting complementarity to RNA of retrovirus of novel human immunodeficient virus of type 2 (HIV-2), by isolating said retrovirus and determining total base sequence of said DNA. CONSTITUTION:From a Ghanian AIDS patient, a retrovirus analogous to HIV-2 but having different antigenicity from HIV-2ROD which had formerly been determined the total base sequence has been isolated and the total base sequence of DNA exhibiting complementarity to RNA of said HIV-2ROD has been determined. In said total base sequence of DNA, existence of XbaI site and HindIII site at only each one position as restricted enzyme site has been made clear and the DNA has been subjected to cloning utilizing said knowledge.