The present invention provides variant strains mass-producing εPL which are obtained by variant treatment of a strain producing εPL. For using the variant strains, the strains are cultured in a cultivated medium, εPL is mass-produced and stored in the culture solution, and the stored εPL is collected from the solution. For producing εPL, a strain which produces εPL is variant-treated, the obtained variant strain is cultivated in a culture medium to which L-lysine or L-lysine and one or more sugars are added, εPL is mass-produced and stored in the culture solution, and the stored εPL is collected from the solution.;The variant strains producing εPL in large quantities are preferably the strains which have tolerance to an analogue of L-lysine of Streptomyces albulus subsp. lysinopolymerus No.346-D strain and plasmid-amplifiable variant strains of the same bacteria.
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