A reusable test unit is disclosed for testing the functionality of a portable blood analyzer, and particularly the interface between the high- impedance electrochemical sensors and the instrument. Signals are generated to simulate amperometric, conductimetric, and potentiometric sensors. Circuitry permits detection and discrimination between failures from damaged CMOS amplifiers and failures from leakage currents in contaminated connectors. The test unit may be configured to be insertable into the instrument to fit mechanically in the same fashion as a disposable sensor device. A scheme for three-dimensional routing of guard lines on a circuit board is disclosed for guarding against coupling between closely space traces and connector pads.
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