CULTURE MEDIUM FOR CULTURING LENTINUS EDODES AND METHOD FOR CULTURING LENTINUS EDODES

摘要

PURPOSE:To obtain the subject culture medium providing large-sized Lentinus edodes (SHIITAKE in Japanese) having high value on the market comprising beet pulp, sawdust and bran by using beet pulp obtained in a process of producing sugar from beet as a nutritive agent and a sawdust substitute. CONSTITUTION:A mixture of beet pulp, sawdust and/or bran having 10 meshes size is mixed with water to prepare a culture medium having about 65wt.% water content, which is packed into a polypropylene bag with a filter and formed into a rectangular parallelepiped. A hole having 20mm diameter is bored through the central part of the culture medium to the bottom part of the bag, the culture medium sterilized at 120 deg.C for 90 minutes, cooled, inoculated with spawn of Lentinus edodes, the mouth of the bag is immediately heat sealed and the spawn is uniformly dispersed into the hole at the central part of the culture medium and the top of a medium. The culture medium after inoculation is cultured at 22 deg.C at 70% humidity for 25 days to grow mycelia and then immersed in water and cultured at 16 deg.C at 90% humidity under 15-300lux illuminance to give large-sized fruit body of Lentinus edodes having high value on the market in a high yield.