Luciferase gene and novel recombinant DNA as well as a method for production of luciferase

摘要

A novel Luciola lateralis-derived luciferase-­coding gene is characterized by the following restric­tion enzyme map: wherein EI represents Eco RI, S represents Ssp I, EV represents Eco RV, A represents Apa I and H represents Hpa I. By inserting the novel gene into vector DNA pUC119, a novel recombinant DNA is provided. A micro­organism from the genus Escherichia bearing the recombi­nant DNA is cultured in a medium and a luciferase is collected from the culture. Luciferase is extremely useful for quantitative determination of ATP.