Thermostabile Luciferase of the light bulb, gene of the thermostatic Luciferase of the light bulb, recombinant DNA, and method of production of the thermostatic Luciferase of the light bulb

摘要

The present invention relates to a thermostable luciferase of a firefly wherein an amino acid at the 217-position of the amino acid sequence of the wild-type firefly luciferase or an amino acid equivalent to the amino acid at the 217-position of the luciferase of GENJI firefly or HEIKE firefly is converted into a hydrophobic amino acid, a gene encoding said thermostable luciferase, a vector comprising the gene encoding said thermostable luciferase inserted therein, and a process for the preparation of a thermostable firefly luciferase comprising the use of said vector.