Litmus paper null for bile Acid measurement

摘要

PURPOSE:To obtain the title test paper which is capable of determining the bile acid in the body fluid accurately, simply in a shortened time, by allowing the carrier made of a macromolecular material to support a specific reagent composition and an additive such as ethylenediaminetetraacetic acid on the same point on the carrier. CONSTITUTION:(A) A carrier of a macromolecular material such as filter paper, nonwoven fabrics, or membrane filter of natural or synthetic fibers (such as a cellulose acetate membrane having 0.1-0.4mu pore diameter) is allowed to support, on the same point, (B) a reagent composition consisting of (a) 3alpha- hydroxysteroid dehydrogenase, 0.1-10,000 IU; (b) nicotinamide adenine dinucleotide, 0.1-100mg; (c) diaphorase, 0.1-10,000 IU and (d) tetrazolium salt, 0.1-500mg, (C) at least one selected from EDTA, ethylene glycol bis(beta- aminoethyl ether)-N,N,N',N'-tetraacetic acid or their alkali metal salts, 1-10,000mg, per 100cm2 of the carrier.