Novel bacterial plasmid shuttle vectors for streptomycetes and escherichia coli

摘要

Four new Escherichiacoli - Streptomyces shuttle vectors are described. These shuttle vectors are useful for cloning both in Streptomyces and in E. coli, and for a variety of applications in molecular genetics technology in Streptomyces. Constructs are structurally stable and replicate efficiently in E. coli and in a number of Streptomyces species, allowing transfer of a wide size range of cloned DNA fragments back and forth from one host to another.