The present invention relates to newly created and isolated insulin-dependent mutant strains of yeast or fungi such as S. cerevisiae. The mutants grow on complex media of defined and undefined compositions. Optimal growth, morphology and viability of certain isolates demonstrate varying dependence upon supraphysiological concentrations of insulin. Other isolates are relatively insensitive to the same concentrations of insulin and can be maintained in the presence of the hormone for extended periods of time without toxic effects. The invention also pertains to a method for detecting insulin secretagogues which employs the mutant yeast cells. Furthermore, the invention encompasses methods for the production of recombinant insulin employing the mutant yeasts as host cells.
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