MODIFIED IMMUNOLOGICALLY ACTIVE SUBSTANCE AND IMMUNOASSAY OF IMMUNOLOGICALLY ACTIVE SUBSTANCE

摘要

PURPOSE: To obtain a modified antibody or antigen, i.e., a modified immunologically active substance, exhibiting high sensitivity and reproducibility in low concentration region by bonding an antibody or an antigen chemically through a polyalkyleneoxide chain. ;CONSTITUTION: The modified immunologically active substance is obtained by bonding 2mol or more of antibody or antigen chemically through a polyalkyleneoxide chain. More specifically, it can be obtained by causing an antibody or antigen to react on a polyoxyalkylene derivative. When a polyoxyalkylene derivative has an end group of hydroxy, the derivative is dissolved into an organic solvent, e.g. 1,4-dioxane, before being caused to react on carbonyldiimidazole. Upon finishing the reaction, the polyoxyalkylene derivative is refined through ultrafiltration or freezing/drying. Finally, it is added with antibody or antigen dissolved into various physiological saline, phosphoric acid buffer solution, etc., thus obtaining a modified immunologically active substance.;COPYRIGHT: (C)1996,JPO