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COMPOSITE BIOLOGICAL-MOLECULE-ACCRETION MATERIAL

机译:复合生物分子积聚材料

摘要

A composite biological-molecule-accretion material (10) is described for extracting desired target biological molecules from aqueous solution (24) and substantially permanently binding to those molecules. The material (10) includes a first component (10a^_) designed with a binding affinity for proteins and peptides in such solution, and a second component (10b^_) designed with a binding affinity for nucleic acids in such solution. The material (10) is capable of being formed in a body (12) for passing such solution through it, and such passing has the effect of removing the proteins, peptides and nucleic acids from such solution and binding them to the body. That binding is substantially permanent so that subsequent passage of additional aqueous solution will not unbind substantially the accreted proteins, peptides and nucleic acids. The first component (10a^_) may include dextran-coated (10a^_2) activated charcoal particles (10a^_1), and the second component may include an anion-exchange resin and a cation-exchange resin. Preferred relative volumes of both components are 1:1. Also described is a method for extracting desired target biological molecules from aqueous solution and substantially permanently binding them to a solid, and a method of filtering target biological molecules out of water.
机译:描述了一种复合生物分子积聚材料(10),用于从水溶液(24)中提取所需的靶生物分子并基本上永久地结合于那些分子。材料(10)包括设计成对这样的溶液中的蛋白质和肽具有结合亲和力的第一组分(10a-3)和设计成对这种溶液中的核酸具有结合亲和力的第二组分(10b -n)。材料(10)能够形成在主体(12)中,以使溶液通过该物质,并且这种通过具有从该溶液中去除蛋白质,肽和核酸并将它们结合到身体上的作用。这种结合基本上是永久性的,因此随后通过另外的水溶液将不会基本上解开结合的蛋白质,肽和核酸。第一组分(10a ^ _)可以包括葡聚糖涂覆的(10a ^ _2)活性炭颗粒(10a ^ _1),并且第二组分可以包括阴离子交换树脂和阳离子交换树脂。两种组分的优选相对体积为1:1。还描述了一种从水溶液中提取期望的靶生物分子并将其基本上永久地结合到固体上的方法,以及从水中过滤出靶生物分子的方法。

著录项

  • 公开/公告号EP0713423A4

    专利类型

  • 公开/公告日1996-09-11

    原文格式PDF

  • 申请/专利权人 ALLEN RICHARD G.;

    申请/专利号EP19940925877

  • 发明设计人 ALLEN RICHARD G.;

    申请日1994-08-12

  • 分类号B01J20/20;

  • 国家 EP

  • 入库时间 2022-08-22 03:46:54

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