Production method of anthraquinones by suspension culture of aloe saponaria

摘要

The present invention provides a method for mass production of anthraquinone by tissue culture of aloe saponaria. The method of the present invention comprises the steps of: (a) culturing leaves of aloe saponaria in a medium supplemented with 10-30 M NA + 5-10 M BA or 10-30 M NAA + 3-7 M kinetin to induce callus; (b) The calli obtained in step (a) were suspended and cultured in a medium supplemented with 25 to 35 M NAA, 5 to 15 M 6-benzylaminopurine (BA) and 0.5% (w / v) polyvinylpyrrolidone, Accumulating quinone; And (c) recovering the anthraquinone from the culture medium. According to the method of the present invention, since the production amount of anthraquinone can be remarkably increased, mass production of anthraquinone having the effect of the dryness and the dressing can be achieved by a simpler method.