Alkaline protease Bacillus sp. JP 395, method of making and detergent compositions

摘要

An alkaline protease from a strain of Bacillus sp. JP 395 has been isolated. The protease has a mass of about 30 kDa as determined by SDS- PAGE, a pH optimum of about 9-11, a temperature optimum of about 50. degree.-65° C., and an isoelectric point above 9.5. The protease is inhibited by PMSF (phenylmethylsulfonylfluoride), but not by EDTA or soybean protein inhibitor. The protease is prepared by growing this strain of bacillus in a suitable media and recovering the protease. The protease has been formulated in detergents and is stable at 40° C. for 60 minutes in these detergents under wash conditions in the presence or absence of bleaches such as perborate.