MULTI-ANGLE, MULTIWAVELENGTH PARTICLE CHARACTERIZATION SYSTEM AND METHOD

摘要

A system and method are provided for the characterization of a sample containing a macromolecule in solution. A light source illuminates the sample, generally in the ultraviolet-visible wavelength range, and a plurality of sensors that are radially disposed about the sample at a plurality of observation angles simultaneously sense the light energy emerging from the sample. An intensity spectrum is calculated as a function of wavelength for each observation angle, from which is calculated a particle characteristic such as shape, conformational change, composition, and particle size distribution. Both scattering and absorption data are utilized to provide complementary information.