A cell culture device (10) comprising a container having a cell culture compartment (40) defined by a lower gas permeable film (120) and an upper sheet (20) selectively permeable to compounds of selected sizes. The device (10) is adapted to allow culture medium (50) to reside between the upper sheet (20) and the lower gas permeable film (120). A basal medium compartment (30) is located above the upper sheet (20) and is adapted to allow basal medium (60) to reside upon the upper sheet (20). Each compartment (30, 40) contains an access port (70, 90). A gas film support (130) below and in partial contact with the gas permeable film (120) holds the gas permeable film (120) in a substantially horizontal position so that suspension or adherent cells can distribute across the surface of the gas permeable film (120).
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