Quantification method and detection (DSP (diarrheic shellfish poison)) of diarrhea-induced crustacean toxin from Uzuben hair algae based on the inhibition of phosphatase

摘要

(57) as well as the quantification method detection (DSP (diarrheic shellfish poison)) of diarrhea induced shellfish toxins from Uzuben hair algae based on the inhibition of [summary] phosphatase. The presence of diarrhea-induced crustacean material generated organisms, cause red tide that contaminate the crustaceans. And its toxicity, before the crustacean is distributed in the market, it is necessary to be constantly Monota. Monitor the current technology is a biological assay, essentially, it is the oral administration of the extract to rats. It is determined The results observed after 12 hours, if mouse dies, to have been contaminated. This method is time consuming, expensive, and are unstable, the selectivity is low. According to the method of the present invention, by measuring the inhibition of the phosphatase, an enzyme that acts poisons, toxins are detected and quantified. Inhibition of this phosphatase is quantified in a microtiter plate. Then, the activity is measured quantitatively by depending on the chemical properties, fluorescence, luminescence, to examine the activity of the enzyme or by using a phosphatase substrate that can change the colorations. Technology of the present invention provides rapidity, low cost, and reliability. This technique is most suitable for canning field of marine industrial.