CULTURE OF ANIMAL CELL USING HOLLOW YARN TYPE INCUBATOR

摘要

PROBLEM TO BE SOLVED: To provide a method for carrying out culture at a prescribed flow rate of cultured medium in an animal cell culture by using a hollow yarn type incubator. ;SOLUTION: This method is to satisfy the formula, 1.5×N×(α/ c)≤(u)≤400×N×(α)/(c), wherein, flow rate of cultured medium fed to the hollow yarn type incubator is defined as (u)ml/min and live cell number of animal cells in the hollow yarn type incubator is defined as N (number), cell constant of the animal cells is defined as (α) and dissolved oxygen concentration of culture solution fed to the hollow yarn type incubator is defined as (c)g/ml.;COPYRIGHT: (C)1998,JPO