METHOD FOR GROWING PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS FOR USE AS VACCINES AND DIAGNOSTIC ASSAYS

摘要

The present invention relates to a method for production of macrophages from monocytes of porcine origin to propagate porcine reproductive and respiratory syndrome virus (PRRSV). Specifically, a specific subset of blood cells from swine called monocytes are isolated and treated with cytokines which render the cells susceptible to PRRSV infection and replication. The method is useful for the production of PRRSV for vaccines and for the replication of PRRSV for diagnostic assays. Furthermore, the method provides for the production of cells susceptible to PRRSV infection that are suitable for the establishment of a continuous cell line for the growth of PRRSV for vaccines.