Lactobacillus delbrueckii Method for Gene Integration into Species Chromosome and Gene Integrant

摘要

A product of gene integration prepared by integrating an arbitrary gene into a chromosomal DNA of a bacillus of Lactobacillus delbrueckii species through a method which comprises constructing an integration plasmid by combining pAM beta 1 plasmid with a target gene, subjecting the obtained plasmid to conjugation transfer and homologous recombination to thereby integrate only the target gene into the chromosomal DNA, and eliminating the DNA sequence of the pAM beta 1 as a vector. The product can be widely applied to the production of food, drug and feed. IMAGE