Gene integration into chromosomes of lactobacillus delbrueckii species and integrants thereof

摘要

A gene to be integrated is bound to pAM&bgr;1 plasmid to construct an integration plasmid, which is conjugally transferred into chromosomal DNA of a strain of Lactobacillus delbrueckii species. Integration of only the objective gene occurs through homologous recombination events (double crossing-over). According to this method, an arbitrary gene can be integrated into chromosomal DNA of a strain of Lactobacillus delbrueckii species and the DNA sequence derived from the vector, pAM&bgr;1, can be completely removed. Therefore, the obtained gene integrant is widely applicable to the production of foods, medicines, feeds and the like.