ENZYMATIC PREPARATION PROCESS OF 7{BETA}(4-CARBOXYBUTANAMIDO)CEPHALOSPORANIC ACID BY MAKING USE OF MODIFIED OXIDASE OF D-AMINO ACIDS FROM TRIGONOPSIS VARIABILIS PRODUCED IN ESCHERICHIA COLI

摘要

An enzymatic process for preparing 7 b acid - (carboxybutanamido) cephalosporanic using the enzyme D-amino acid oxidase of Trigonopsis variabilis produced in Escherichia coli modified. The method of expression of the enzyme comprises: for the enzyme D-amino acid oxidase; (II) removing the intron which contains said gene; (III) inserting the DNA fragment obtained in a plasmid capable of replicating in Escherichia coli (IV) fusing at the 5 '' 'of the structural region of the gene a synthetic assembler which contains a nucleotide sequence coding for six histidines; (V) transforming a strain of Escherichia coli with the resulting recombinant plasmid; (VI) cultivating Escherichia coli cells transformed and (VII) recovering the enzyme D-amino acid oxidase crop of the previous operation by affinity chromatography.