The present invention relates to an electrochemical immunobiosensor which comprises an electrode part where a plurality of electrodes are formed on an insulating base plate and a part of the electrode is exposed by selectively forming an insulating layer on the electrode; and a matrix part where a portion absorbed with analyte-cytolytic agent conjugates, a portion with an immobilized recepter which binds with the analyte, and a portion fixed with electroactive species-containing liposomes, to perform the functions of liposome-based signal amplification and immunochromatography; and said electrode portion exposed in the electrode part and the liposome portion in the matrix are integrally combined.
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