XYLOSEISOMERASE GENE OF THERMUS AQUATICUS, XYLOSEISOMERASE AND PROCESS FOR PREPARATION OF FRUCTOSE

摘要

A xylose isomerase gene of Termus Aquaticus, a xyl isomerase of Termus Aquaticus, and a method for producing this xylose isomerase are provided. Also provided is a method of producing the xylose isomerase obtained without coloring a high concentration of plotose solution.;Isolation, identification and cloning of the xylose isomerase gene of Termus Aquaticus, a type of hyperthermic bacteria. Xylose isomerase obtained by culturing a microorganism transformed with the plasmid having the xylose isomerase gene obtained by cloning and a neutron is genetically prepared. The obtained xylose isomerase has an intellectual temperature of about 95 ° C., is thermally stabilized by magnesium, and is different from the natural xylose isomerase of Termus aquatiscus. In addition, glucose isomerized at a temperature of 75 ° C. or higher and at 5 to 7 pH under the preparation of Ixylose isomerase to prepare a solution having a high lactose concentration.