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Method for biotechnological production of ligand binding, especially extracellular, fragments of receptor subunits, especially neuroreceptors and hormone receptors in isolated native form
Method for biotechnological production of ligand binding, especially extracellular, fragments of receptor subunits, especially neuroreceptors and hormone receptors in isolated native form
The invention relates inter alia to a method for the biotechnological production of ligand binding fragments of subunits of neuroreceptors and hormone receptors in isolated native form. Said method comprises the following steps: expression of the fragments; solubilization of the fragments, especially those contained in inclusion bodies; removal of the denaturing agent required for solubilization; renaturation of the fragments and removal of the agent required for renaturation, wherein the neuroreceptors are from the supergene family of ligand regulated pentameric ion channels.
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