QUANTITATIVE POLYMERASE CHAIN REACTION USING A FLUOROGENIC REAL-TIME DETECTION SYSTEM

摘要

The present invention concerns an improved quantitative one-tube fluorogenic real-time polymerase chain reaction. The improvement exists in the use of very specific probes allowing much more reliable and fast analysis, since they allow the selective analysis of various pathogens and mRNAs coding for cytokines and other proteins. The improvement is obtained by using probes selectively hybridizing with the pathogens of interest.