E.COLI JM38 STRAIN COMPRISING HALOPHILIC AGARASE GENE PLASMID, AND AGARASE

摘要

[Problem Solving Invention];There are many seaweeds in the ocean that can be used for industrial purposes or food. Especially, agar has been used as an important raw material in various industries including food and medicine for a long time, but it is sold without processing at all. Therefore, agar or electrophoretic agarose, which is used as a test medium, is imported at a very high price. Therefore, in the present invention, E. coli JM83 (pSW3) having agar degrading enzyme production ability was selected from marine microorganisms having agar degrading ability, and then separated and purified agarases produced by this strain, and mass production was attempted to activate domestic related industries. At the same time, it contributes to economic and industrial development by converting agar, which has been used as a cheap raw material, to high value-added materials.;[Solution];The N-terminal amino acid sequence of the isolated protein was recombined with the Agarase gene of Met-Lys-Arg-Ala-Phe-lle-Met-Val-Leu-Asp and Halophilic Pseudomonas sp.W7. Method for the preparation of agarase enzyme having N-terminal amino acid sequence produced by E. coli JM83 (pSW3) strain containing plasmid Met-Lys-Arg-Ala-Phe-lle-Met-Val-Leu-Asp