CHLAMYDIA TRACHOMATIS SPECIFIC PEPTIDES AND THEIR USE IN DIAGNOSTIC ASSAYS

摘要

Peptides or a mixture of peptides derived from the variable domains of the Chlamydia trachomatis (C. trachomatis) immunodominant major outer membrane protein (MOMP), said peptides or mixture of peptides characterized by having specificity only to C. trachomatis anti- MOMP antibodies and being non-cross reactive with anti-MOMP antibodies of other chlamydia species and said mixture also characterized by having specificity to anti-MOMP antibodies from all serovars of C. trachomatis, said peptides selected from: (a) SEQ ID No. 1l herein also designated peptide 4A, having the amino acid sequence: I F D T T L N P T I A G A G D V K; (b) SEQ ID No. 2, herein also designated peptide 4B having the amino acid sequence: V D I T T L N P T I A G C G S V A K; (c) SEQ ID No. 3, herein also designated peptide 4C, having the amino acid sequence: C V F D V T T L N P T I A G A G D V K; (d) SEQ ID No. 4, herein also designated peptide 4D, having the amino acid sequence: L A E A I L D V T T L N P T I T G K A V V S K; wherein all of said peptides (a)-(d) are derived from the fourth variable domain (VDIV) of said MOMP: (e) SEQ ID NO. 5, herein also designated peptide C.t2A, having the amino acid sequence: C D N E N Q S T V K T N S V P N M S L D Q S K, wherein said peptide (e) is derived from the second variable domain (VDII) of said MOMP; (f) analogs of any one of peptides (a)-(e), wherein said analogs have essentially the same biological activity as said peptides (a)-(e), said analogs having between about 17 to about 23 amino acid residues and differing from said peptides (a)-(e) by having one or more additions, deletions or substitutions, or by being combinations of an N-terminal part of one peptide selected from (a) to (d) with a C-terminal part of another peptide selected from (a) to (d), such that the complete sequence will be homologous to the VDIV sequence. 1328 כ" ג בסיון התשס" א - June 14, 2001