Method for reducing phytate in canola meal using genetic manipulation involving myo-inositol 1-phosphate synthase gene

摘要

A plant, multicellular fragment of said plant or seed of said plant transformed with a nucleotide sequence of SEQ ID NO 1 or an allelic variant or a fragment thereof or a genetic equivalent thereof according to the degeneracy of the genetic code coding for a peptide having a uBrassica/u myo-inositol 1-phosphate synthase activity, said plant, multicellular fragment or seed having reduced myo-inositol 1-phosphate synthase activity when compared with an equivalent untransformed plant, multicellular fragment or seed, such that there is reduced phytate present in the plant, multicellular fragment or seed. The invention also provides a method for reducing phytate in uBrassica/u, which method comprises growing a uBrassica/u plant comprising one of a myo-inositol 1-phosphate synthase antisense sequence and a myo-inositol 1-phosphate synthase cosuppression sequence thereby yielding a reduced amount of myo-inositol 1-phosphate synthase and consequently reduced phytate in said uBrassica/u.