METHOD FOR REDUCING PHYTATE IN CANOLA MEAL USING GENETIC MANIPULATION INVOLVING MYO-INOSITOL 1-PHOSPHATE SYNTHASE GENE

摘要

A plant, multicellular fragment of said plant or seed of said planttransformed with a nucleotide sequence of SEQ ID NO 1 or an allelic variant ora fragment thereof or a genetic equivalent thereof according to the degeneracyof the genetic code coding for a peptide having a uBrassica/u myo-inositol1-phosphate synthase activity, said plant, multicellular fragment or seedhaving reduced myo-inositol 1-phosphate synthase activity when compared withan equivalent untransformed plant, multicellular fragment or seed, such thatthere is reduced phytate present in the plant, multicellular fragment or seed.The invention also provides a method for reducing phytate in uBrassica/u,which method comprises growing a uBrassica/u plant comprising one of a myo-inositol 1-phosphate synthase antisense sequence and a myo-inositol 1-phosphate synthase cosuppression sequence thereby yielding a reduced amount ofmyo-inositol 1-phosphate synthase and consequently reduced phytate in saiduBrassica/u.