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METHOD FOR PREPARING A MODEL SYSTEM FOR CELLULAR INSULIN RESISTANCE AND DEVICE FOR USE WITH THE MODEL SYSTEM
METHOD FOR PREPARING A MODEL SYSTEM FOR CELLULAR INSULIN RESISTANCE AND DEVICE FOR USE WITH THE MODEL SYSTEM
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机译:制备细胞胰岛素抵抗模型系统的方法及与该模型系统一起使用的装置
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摘要
A method of preparing a cellular in vitro model system for insulin resistance by inducing insulin resistance to an animal cell culture in a cell culture medium comprises incubating the cell culture in the presence of glucose and at east one fatty acid, preferably a long-chain fatty acid, wherein the concentration of glucose is in the range of about 5 to about 25 mM and the concentration of fatty acid is less than about 2 mM. A device that may be used in the method comprises a cell culture flask (1), and a support member (2) for a carbon dioxide absorbent body, which support member (2) is partially insertable into the culture flask (1) and fixable in the flask opening with the absorbent body extending into the flask.
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