Method for preparing a model system for cellular insulin resistance and device for use with the model system

摘要

A method of preparing a cellular in vitro model system for insulin resistance by inducing insulin resistance to an animal cell culture in a cell culture medium comprises incubating the cell culture in the presence of glucose and at east one fatty acid, preferably a long-chain fatty acid, wherein the concentration of glucose is in the range of about 5 to about 25 mM and the concentration of fatty acid is less than about 2 mM. A device that may be used in the method comprises a cell culture flask (1), and a support member (2) for a carbon dioxide absorbent body, which support member (2) is partially insertable into the culture flask (1) and fixable in the flask opening with the absorbent body extending into the flask.